目的:探讨雌二醇E2通过调控色素上皮细胞衍生因子PEDF促进乳腺癌增殖的分子机制。方法:ELISA法检测血清中PEDF水平;放射免疫分析法检测雌二醇E2水平;Western blot检测雌二醇对PEDF调控作用;分子克隆过表达PEDF研究其在雌二醇E2促进乳腺癌增殖中作用;MTT法检测乳腺癌细胞株MCF-7的增殖能力。结果:乳腺癌中PEDF表达下调,且与雌二醇E2水平呈负相关(R = −0.69; ***P < 0.001);雌二醇E2可下调PEDF的表达,促进乳腺癌细胞MCF-7的增殖。结论:雌二醇E2通过调控PEDF表达进而促进乳腺癌的增殖。 Objective: To investigate the molecular mechanism of estradiol E2 to promote the proliferation of breast cancer by regulating the expression PEDF. Methods: Serum PEDF level was detected by ELISA kit and estradiol E2 level was detected by radioimmunoassay; the effect of estradiol on the PEDF regulation was detected by Western blot; the proliferation of breast cancer cell line MCF-7 was detected by MTT assay. Results: The expression of PEDF was down regulated in breast cancer, and negatively correlated with estradiol E2 level. Estradiol E2 can reduce the expression of PEDF and promote the proliferation of breast cancer cell MCF-7. Conclusion: Estradiol E2 can promote the proliferation of breast cancer by regulating the expression of PEDF.
乳腺癌,雌二醇E2,色素上皮细胞衍生因子PEDF,增殖, Breast Cancer Estradiol E2 Pigment Epithelial Cell Derived Factor PEDF Proliferation雌二醇E2通过调控PEDF表达促进乳腺癌增殖作用研究
洪宏海,杨卫琴,唐希才,冯 遥. 雌二醇E2通过调控PEDF表达促进乳腺癌增殖作用研究The Effect of Estradiol E2 on the Proliferation of Breast Cancer by Regulating PEDF Expression[J]. 世界肿瘤研究, 2018, 08(01): 49-54. http://dx.doi.org/10.12677/WJCR.2018.81008
参考文献 (References)ReferencesPatel, H.K. and Bihani, T. (2017) Selective Estrogen Receptor Modulators (SERMS) and Selective Estrogen Receptor Degraders (SERDs) in Cancer Treatment. Pharmacology & Therapeutics, in Press.
<br>https://doi.org/10.1016/j.pharmthera.2017.12.012Patel, S. (2017) Breast Cancer: Lesser-Known Facets and Hypotheses. Biomedicine & Pharmacotherapy, 98, 499-506.
<br>https://doi.org/10.1016/j.biopha.2017.12.087Weigelt, B., Peterse, J.L. and van’t Veer, L.J. (2005) Breast Cancer Metastasis: Markers and Models. Nature Reviews Cancer, 5, 591-602. <br>https://doi.org/10.1038/nrc1670Kodack, D.P., et al. (2015) Emerging Strategies for Treating Brain Metastases from Breast Cancer. Cancer Cell, 27, 163-175. <br>https://doi.org/10.1016/j.ccell.2015.01.001Majumder, A., Singh, M. and Tyagi, S.C. (2017) Post-Menopausal Breast Cancer: From Estrogen to Androgen Receptor. Oncotarget, 8, 102739-102758. <br>https://doi.org/10.18632/oncotarget.22156Bilak, M.M., et al. (1999) Pigment Ep-ithelium-Derived Factor (PEDF) Protects Motor Neurons from Chronic Glutamate-Mediated Neurodegeneration. Journal of Neuropathology & Experimental Neurology, 58, 719-728.
<br>https://doi.org/10.1097/00005072-199907000-00006Becerra, S.P. (2006) Focus on Molecules: Pigment Ep-ithelium-Derived Factor (PEDF). Experimental Eye Research, 82, 739-740. <br>https://doi.org/10.1016/j.exer.2005.10.016Hong, H., Zhou, T., Fang, S., et al. (2014) Pigment Epithe-lium-Derived Factor (PEDF) Inhibits Breast Cancer Metastasis by Down-Regulating Fibronectin. Breast Cancer Re-search and Treatment, 148, 61-72.
<br>https://doi.org/10.1007/s10549-014-3154-9Bae, Y.K., et al. (2013) Fibronectin Expression in Carcinoma Cells Correlates with Tumor Aggressiveness and Poor Clinical Outcome in Patients with Invasive Breast Cancer. Human Pathology, 44, 2028-2037.
<br>https://doi.org/10.1016/j.humpath.2013.03.006Sawant, S., et al. (2004) Regulation of Factors Controlling Angiogenesis in Liver Development: A Role for PEDF in the Formation and Maintenance of Normal Vasculature. Bi-ochemical and Biophysical Research Communications, 325, 408-413. <br>https://doi.org/10.1016/j.bbrc.2004.10.041Filleur, S., et al. (2009) Characterization of PEDF: A Mul-ti-Functional Serpin Family Protein. Journal of Cellular Biochemistry, 106, 769-775. <br>https://doi.org/10.1002/jcb.22072Bouck, N. (2002) PEDF: Anti-Angiogenic Guardian of Ocular Function. Trends in Molecular Medicine, 8, 330-334.
<br>https://doi.org/10.1016/S1471-4914(02)02362-6Cai, J., et al. (2006) Decreased Pigment Epithelium-Derived Factor Expression in Human Breast Cancer Progression. Clinical Cancer Research, 12, 3510-3517. <br>https://doi.org/10.1158/1078-0432.CCR-06-0094