微囊藻水华可产生微囊藻毒素,严重威胁生态系统。人类活动的日益加剧使得大量的氮、磷等营养盐排入淡水水体,加剧了水华的爆发。本文综述了国内外微囊藻毒素的产生及其危害和去除方法,重点关注了其分析方法和未来可能的研究方向。
The metabolites produced by the Microcystis blooms are called microcystins and pose a great threat to the ecosystem. Industrial and agricultural activities cause a large amount of nitrogen and phosphorus nutrients to be discharged into freshwater bodies and leading to the outbreak of water blooms. This review summarizes the research status on the generation, ecotoxicology and removal methods of microcystins at home and abroad, especially focusing on analysis method and proposes prospects for future research perspective.
微囊藻毒素,研究现状,生态风险,去除方法, Microcystins Research Status Ecotoxicity Removal Method微囊藻毒素的危害及其分析方法进展
钟 力,徐文琦,胡静文,张大海. 微囊藻毒素的危害及其分析方法进展 The Harmful Effects of Microcystins and the Analytical Methods[J]. 分析化学进展, 2018, 08(03): 91-102. https://doi.org/10.12677/AAC.2018.83012
参考文献ReferencesMatthiensen, A., Beattie, K.A., Yunes, J.S., et al. (2000) [D-Leu1] Microcystin-LR, from the Cyanobacterium Microcystis RST 9501 and from a Microcystis Bloom in the Patos Lagoon Estuary, Brazil. Phytochemistry, 55, 383-387.
https://doi.org/10.1016/S0031-9422(00)00335-6王扬才, 陆开宏. 蓝藻水华的危害及治理动态[J]. 水产学杂志, 2004, 17(1): 90-94.许川, 舒为群. 微囊藻毒素污染状况、检测及其毒效应[J]. 国外医学: 卫生学分册, 2005, 32(1): 56-60.Chen, J. and Xie, P. (2007) Microcystin Accumulation in Freshwater Bivalves from Lake Taihu, China, and the Potential Risk to Human Consumption. Environmental Toxicology & Chemistry, 26, 1066-1073.
https://doi.org/10.1897/06-423R1.1Jiang, Y., Xie, P. and Nie, Y. (2014) Concentration and Bioaccumulation of Cyano-bacterial Bioactive and Odorous Metabolites Occurred in a Large, Shallow Chinese Lake. Bulletin of Environmental Contamination & Toxicology, 93, 643. https://doi.org/10.1007/s00128-014-1350-2Wu, S., Wang, S., Yang, H., et al. (2008) Field Studies on the Environmental Factors in Controlling Microcystin Production in the Subtropical Shallow Lakes of the Yangtze River. Bulletin of Environmental Contamination & Toxicology, 80, 329-334. https://doi.org/10.1007/s00128-008-9378-9Carmichael, W.W. (1996) Toxic Microcystis and the Environment. Toxic Microcystis.Orr, P.T. and Jones, G.J. (1998) Relationship between Microcystin Production and Cell Division Rates in Nitrogen-Limited Microcystis aeruginosa Cultures. Limnology & Oceanography, 43, 1604-1614.
https://doi.org/10.4319/lo.1998.43.7.1604Singh, D.P., Tyagi, M.B., Kumar, A., et al. (2001) Antialgal Activity of a Hepatotoxin-Producing Cyanobacterium, Microcystis aeruginosa. World Journal of Microbiology & Biotechnology, 17, 15-22.
https://doi.org/10.1023/A:1016622414140Dawson, R.M. (1998) The Toxicology of Microcystins. Toxicon, 36, 953-962.
https://doi.org/10.1016/S0041-0101(97)00102-5Lindholm, T., Öhman, P., Kurkihelasmo, K., et al. (1999) Toxic Algae and Fish Mortality in a Brackish-Water Lake in Åland, SW Finland. Hydrobiologia, 397, 109-120. https://doi.org/10.1023/A:1003667728458Codd, G.A. and Zalewski, M. (2000) Cyanobacterial Toxins, the Perception of Water Quality, and the Prioritization of Eutrophication Control. Ecological Engineering, 16, 51-60. https://doi.org/10.1016/S0925-8574(00)00089-6Falconer, I.R., Beresford, A.M. and Runnegar, M.T. (1983) Evidence of Liver Damage by Toxin from a Bloom of the Blue-Green Alga, Microcystis aeruginosa. Medical Journal of Australia, 1, 511.Howard, A., Mcdonald, A.T., Kneale, P.E., et al. (1996) Cyanobacterial (Blue-Green Algal) Blooms in the UK: A Review of the Current Situation and Potential Management Options. Progress in Physical Geography, 20, 53-61.
https://doi.org/10.1177/030913339602000103Pouria, S., De, A.A., Barbosa, J., et al. (1998) Fatal Microcystin Intoxication in Haemodialysis Unit in Caruaru, Brazil. Lancet, 352, 21-26. https://doi.org/10.1016/S0140-6736(97)12285-1Ueno, Y., Nagata, S., Tsutsumi, T., et al. (1996) Detection of Microcystins, a Blue-Green Algal Hepatotoxin, in Drinking Water Sampled in Haimen and Fusui, Endemic Areas of Primary Liver Cancer in China, by Highly Sensitive Immunoassay. Carcinogenesis, 17, 1317-1321. https://doi.org/10.1093/carcin/17.6.1317Yu, S.-Z. (1995) Primary Prevention of Hepatocellular Carcinoma. Journal of Gastroenterology & Hepatology, 10, 674-682. https://doi.org/10.1111/j.1440-1746.1995.tb01370.x中华人民共和国卫生部卫生法制与监督司. 生活饮用水卫生规范[M]. 2001.中华人民共和国住房和城乡建设部. 城市供水水质标准(CJ/T206-2005)[M]. 2005.Kleinkauf, H. and Von Döhren, H. (1996) A Nonribosomal System of Peptide Biosynthesis. Eu-ropean Journal of Biochemistry, 236, 335-351. https://doi.org/10.1111/j.1432-1033.1996.00335.xPreece, E.P., Hardy, F.J., Moore, B.C., et al. (2017) A Review of Microcystin Detections in Estuarine and Marine Waters: Environmental Implications and Human Health Risk. Harmful Algae, 61, 31-45.
https://doi.org/10.1016/j.hal.2016.11.006Humble, A.V., Gadd, G.M. and Codd, G.A. (1997) Binding of Copper and Zinc to Three Cyanobacterial Microcystins Quantified by Differential Pulse Polarography. Water Research, 31, 1679-1686.
https://doi.org/10.1016/S0043-1354(97)00033-XUtkilen, H. and Gjølme, N. (1995) Iron-Stimulated Toxin Production in Microcystis aeruginosa. Applied & Environmental Microbiology, 61, 797-800.Dai, R., Liu, H., Qu, J., et al. (2008) Relationship of Energy Charge and Toxin Content of Microcystis aeruginosa in Nitrogen-Limited or Phosphorous-Limited Cultures. Toxicon, 51, 649-658.
https://doi.org/10.1016/j.toxicon.2007.11.021Gorham, P.R. (1964) Toxic Algae. In: Jackson, D.F., Eds., Algae and Man, Springer, Boston, MA, 307-336.
https://doi.org/10.1007/978-1-4684-1719-7_15Watanabe, M.F. and Oishi, S. (1985) Effects of Environmental Factors on Toxicity of a Cyanobacterium (Microcystis aeruginosa) under Culture Conditions. Applied & Environmental Microbiology, 49, 1342-1344.Westhuizen, A.J.V.D. and Eloff, J.N. (1985) Effect of Temperature and Light on the Toxicity and Growth of the Blue-Green Alga Microcystis aeruginosa, (UV-006). Planta, 163, 55-59. https://doi.org/10.1007/BF00395897Utkilen, H. and Gjolme, N. (1998) Energy the Dominating Controlling Factor for Microcystin Production in Microcystis aeruginosa. Compilation of Abstracts. 4th International Conference on Toxic Cyanobacteria, No. 63.Botes, D.P., Wessels, P.L., Kruger, H., et al. (1986) ChemInform Abstract: Structural Studies on Cyanoginosins-LR, -YR, -YA, and -YM, Peptide Toxins from Microcystis aeruginosa. Cheminform, 17, 2747-2748.
https://doi.org/10.1002/chin.198617354Carmichael, W.W., Beasley, V., Bunner, D.L., et al. (1988) Naming of Cyclic Heptapeptide Toxins of Cyanobacteria (Blue-Green Algae). Toxicon, 26, 971-973. https://doi.org/10.1016/0041-0101(88)90195-XAa, D.L.C., Antoniou, M.G., Hiskia, A., et al. (2011) Can We Effectively Degrade Microcystins?—Implications on Human Health. Anti-Cancer Agents in Medicinal Chemistry, 11, 19-37. https://doi.org/10.2174/187152011794941217Rinehart, K.L., Namikoshi, M. and Choi, B.W. (1994) Structure and Bio-synthesis of Toxins from Blue-Green Algae (Cyanobacteria). Journal of Applied Phycology, 6, 159-176. https://doi.org/10.1007/BF02186070Rivasseau, C., Martins, S. and Hennion, M.C. (1998) Determination of Some Physi-cochemical Parameters of Microcystins (Cyanobacterial Toxins) and Trace Level Analysis in Environmental Samples Using Liquid Chromatography. Journal of Chromatography A, 799, 155. https://doi.org/10.1016/S0021-9673(97)01095-9Maagd, G.J.D., Hendriks, A.J., Seinen, W., et al. (1999) pH-Dependent hydrophobicity of the Cyanobacteria Toxin Microcystin-LR. Water Research, 33, 677-680. https://doi.org/10.1016/S0043-1354(98)00258-9Namikoshi, M., Rinehart, K.L., Dahlem, A.M., et al. (1989) Total Synthesis of Adda, the Unique C20, Amino Acid of Cyanobacterial Hepatotoxins. Tetrahedron Letters, 30, 4349-4352. https://doi.org/10.1016/S0040-4039(00)99357-2An, J. and Carmichael, W.W. (1994) Use of a Colorimetric Protein Phosphatase Inhibition Assay and Enzyme Linked Immunosorbent Assay for the Study of Microcystins and Nodularins. Toxicon, 32, 1495-1507.
https://doi.org/10.1016/0041-0101(94)90308-5Ito, E., Harada, K. and Kondo, F. (2000) First Report on the Distribution of Orally Administered Microcystin-LR in Mouse Tissue Using an Immunostaining Method. Toxicon, 38, 37-48. https://doi.org/10.1016/S0041-0101(99)00084-7Nishiwaki-Matsushima, R., Ohta, T., Nishiwaki, S., et al. (1992) Liver Tumor Promotion by the Cyanobacterial Cyclic Peptide Toxin Microcystin-LR. Journal of Cancer Research and Clinical Oncology, 118, 420-424.
https://doi.org/10.1007/BF01629424Miura, G.A., Robinson, N.A., Lawrence, W.B., et al. (1991) Hepatotoxicity of Mi-crocystin-LR in Fed and Fasted Rats. Toxicon, 29, 337-346. https://doi.org/10.1016/0041-0101(91)90287-2Eriksson, J.E., Grönberg, L., Nygård, S., et al. (1990) Hepatocellular Uptake of 3H-Dihydromicrocystin-LR, a Cyclic Peptide Toxin. Biochimica et Biophysica Acta, 1025, 60-66. https://doi.org/10.1016/0005-2736(90)90190-YMacías-Silva, M. and García-Sáinz, J.A. (1994) Inhibition of Hormone-Stimulated Inositol Phosphate Production and Disruption of Cytoskeletal Structure. Effects of Okadaic Acid, Microcystin, Chlorpromazine, W7 and Nystatin. Toxicon, 32, 105-112. https://doi.org/10.1016/0041-0101(94)90026-4Funk, C.D. (2001) Prostaglandins and Leukotrienes: Advances in Eico-sanoid Biology. Science, 294, 1871-1875.
https://doi.org/10.1126/science.294.5548.1871Naseem, S.M., Hines, H.B. and Creasia, D.A. (1990) Inhibition of Micro-cystin-Induced Release of Cyclooxygenase Products from Rat Hepatocytes by Anti-Inflammatory Steroids. Proceedings of the Society for Experimental Biology & Medicine Society for Experimental Biology & Medicine, 195, 345-349. https://doi.org/10.3181/00379727-195-43153Tracey, K.J., Beutler, B., Lowry, S.F., et al. (1986) Shock and Tissue Injury Induced by Recombinant Human Cachectin. Science, 234, 470-474. https://doi.org/10.1126/science.3764421Bhattacharya, R., Sugendran, K., Dangi, R.S., et al. (1997) Toxicity Evaluation of Freshwater Cyanobacterium Microcystis aeruginosa PCC 7806: II. Nephrotoxicity in Rats. Biomedical and Environment Sciences, 10, 93-101.Milutinović, A., Zivin, M., Zorc-Pleskovic, R., et al. (2003) Nephrotoxic Effects of Chronic Administration of Microcystins-LR and -YR. Toxicon, 42, 281-288. https://doi.org/10.1016/S0041-0101(03)00143-0Nobre, A.C.L., Coêlho, G.R., Coutinho, M.C.M., et al. (2001) The Role of Phospholipase A(2) and Cyclooxygenase in Renal Toxicity Induced by Microcystin-LR. Toxicon, 39, 721-724. https://doi.org/10.1016/S0041-0101(00)00193-8Rao, P.V. and Bhattacharya, R. (1996) The Cyanobacterial Toxin Mi-crocystin-LR Induced DNA Damage in Mouse Liver in Vivo. Toxicology, 114, 29-36. https://doi.org/10.1016/S0300-483X(96)03413-0Lankoff, A., Bialczyk, J., Dziga, D., et al. (2006) The Repair of Gam-ma-Radiation-Induced DNA Damage Is Inhibited by Microcystin-LR, the PP1 and PP2A Phosphatase Inhibitor. Mutagenesis, 21, 83-90.
https://doi.org/10.1093/mutage/gel002Donati, C., Drikas, M., Hayes, R. and Newcombe, G. (1994) Microcystin-LR Ad-sorption by Powdered Activated Carbon. Water Research, 28, 1735-1742. https://doi.org/10.1016/0043-1354(94)90245-3Lahti, K., Rapala, J., Kivimäki, A.L., et al. (2001) Occurrence of Micro-cystins in Raw Water Sources and Treated Drinking Water of Finnish Waterworks. Water Science & Technology, 43, 225-228.
https://doi.org/10.2166/wst.2001.0744Fawell, J.K., Hart, J., James, H.A., et al. (1993) Blue-Green Algae and Their Tox-ins—Analysis, Toxicity, Treatment and Environmental Control. Water Supply, 11, 109-121.Pendleton, P., Schumann, R. and Wong, S.H. (2001) Microcystin-LR Adsorption by Activated Carbon. Journal of Colloid and Interface Science, 240, 1-8. https://doi.org/10.1006/jcis.2001.7616Pendleton, P., Schumann, R. and Wong, S.H. (2002) Microcystin-RR Adsorption by Activated Carbon. Journal of Colloid and Interface Science, 24, 11-18.Wang, H., Ho, L., Lewis, D.M., et al. (2007) Dis-criminating and Assessing Adsorption and Biodegradation Removal Mechanisms during Granular Activated Carbon Filtration of Microcystin Toxins. Water Research, 41, 4262-4270.
https://doi.org/10.1016/j.watres.2007.05.057Ge, S., Liu, W., Lei, G., et al. (2013) In Situ Assembly of Porous Au-Paper Electrode and Functionalization of Magnetic Silica Nanoparticles with HRP via Click Chemistry for Microcystin-LR Immunoassay. Biosensors & Bioelectronics, 49, 111-117. https://doi.org/10.1016/j.bios.2013.05.010Iwamoto, M., Hirata, J.I., Matsukami, K. and Kagawa, S. (1983) ChemInform Abstract: Catalytic Oxidation by Oxide Radical Ions. 1. One-Step Hydroxylation of Benzene to Phenol over Group 5 and 6 Oxides Supported on Silica Gel. Chemischer Informationsdienst, 14, 903-905. https://doi.org/10.1002/chin.198327092Morris, R.J., Williams, D.E., Luu, H.A., et al. (2000) The Adsorption of Micro-cystin-LR by Natural Clay Particles. Toxicon, 38, 303-308. https://doi.org/10.1016/S0041-0101(99)00149-XSun, X., Guan, L., Shi, H., et al. (2013) Determination of Microcystin-LR with a Glassy Carbon Impedimetric Immunoelectrode Modified with an Ionic Liquid and Multiwalled Carbon Nanotubes. Microchimica Acta, 180, 75-83.
https://doi.org/10.1007/s00604-012-0912-4Lawton, L.A., Cornish, B.J.P.A. and Macdonald, A.W.R. (1998) Removal of Cyanobacterial Toxins (Microcystins) and Cyanobacterial Cells from Drinking Water Using Domestic Water Filters. Water Research, 32, 633-638.
https://doi.org/10.1016/S0043-1354(97)00267-4Lambert, T.W., Holmes, C.F.B. and Hrudey, S.E. (1996) Adsorption of Microcystin-LR by Activated Carbon and Removal in Full Scale Water Treatment. Water Research, 30, 1411-1422. https://doi.org/10.1016/0043-1354(96)00026-7Neumann, U. and Weckesser, J. (2015) Elimination of Microcystin Peptide Toxins from Water by Reverse Osmosis. Environmental Toxicology, 13, 143-148.Qu, F., Liang, H., Wang, Z., et al. (2012) Ultrafiltration Membrane Fouling by Extracellular Organic Matters (EOM) of Microcystis aeruginosa in Stationary Phase: Influences of Interfacial Characteristics of Foulants and Fouling Mechanisms. Water Research, 46, 1490-1500. https://doi.org/10.1016/j.watres.2011.11.051Hoeger, S.J., Dietrich, D.R. and Hitzfeld, B.C. (2002) Effect of Ozonation on the Removal of Cyanobacterial Toxins during Drinking Water Treatment. Environmental Health Perspectives, 110, 1127-1132.Rositano, J., Newcombe, G., Nicholson, B. and Sztajnbok, P. (2001) Ozonation of NOM and Algal Toxins in Four Treated Waters. Water Research, 35, 23-32. https://doi.org/10.1016/S0043-1354(00)00252-9Nicholson, B.C., Rositano, J. and Burch, M.D. (1994) Destruction of Cyanobacterial Peptide Hepatotoxins by Chlorine and Chloramine. Water Research, 28, 1297-1303. https://doi.org/10.1016/0043-1354(94)90294-1Watanabe, M.F., Tsuji, K., Watanabe, Y., et al. (1992) Release of Heptapeptide Toxin (Microcystin) during the Decomposition Process of Microcystis aeruginosa. Natural Toxins, 1, 48-53. https://doi.org/10.1002/nt.2620010110Welker, M. and Steinberg, C. (2000) Rates of Humic Substance Photosensitized Degradation of Microcystin-LR in Natural Waters. Environmental Science & Technology, 34, 3415-3419. https://doi.org/10.1021/es991274tTsuji, K., Watanuki, T., Kondo, F., et al. (1996) Stability of Microcystins from Cyano-bacteria. II. Effect of UV Light on Decomposition and Isomerization. Toxicon, 33, 1619-1631. https://doi.org/10.1016/0041-0101(95)00101-8Wörmer, L., Huertafontela, M., Cirés, S., et al. (2010) Natural Photodeg-radation of the Cyanobacterial Toxins Microcystin and Cylindrospermopsin. Environmental Science & Technology, 44, 3002-3007.
https://doi.org/10.1021/es9036012Burns, J.M., Cooper, W.J., Ferry, J.L., et al. (2012) Methods for Reactive Oxygen Species (ROS) Detection in Aqueous Environments. Aquatic Sciences, 74, 683-734. https://doi.org/10.1007/s00027-012-0251-x陈伟, 甘南琴, 宋立荣. 微囊藻毒素在单波长紫外光照射下的光降解动态研究[J]. 化学学报, 2004, 62(2): 142-147.李伟英, 许京晶, 张明, 等. 紫外光对微囊藻毒素-LR的去除效果研究[J]. 中国给水排水, 2010, 26(17): 62-65.Sharma, V.K., Triantis, T.M., Antoniou, M.G., et al. (2012) Destruction of Microcystins by Conventional and Advanced Oxidation Processes: A Review. Separation & Purification Technology, 91, 3-17.
https://doi.org/10.1016/j.seppur.2012.02.018Lawton, L.A., Robertson, P.K.J., Cornish, B., et al. (1999) Detoxification of Microcystins (Cyanobacterial Hepatotoxins) Using TiO2 Photocatalytic Oxidation. Environmental Science & Technology, 33, 771-775.
https://doi.org/10.1021/es9806682Antoniou, M.G., Nicolaou, P.A., Shoemaker, J.A., et al. (2009) Impact of the Mor-phological Properties of Thin TiO2 Photocatalytic Films on the Detoxification of Water Contaminated with the Cyanotoxin, Micro-cystin-LR. Applied Catalysis B: Environmental, 91, 165-173. https://doi.org/10.1016/j.apcatb.2009.05.020Hamilton, J.W.J., Byrne, J.A., Dunlop, P.S.M., et al. (2014) Evaluating the Mechanism of Visible Light Activity for N,F-TiO2 Using Photoe-lectrochemistry. Journal of Physical Chemistry C, 118, 12206-12215.
https://doi.org/10.1021/jp4120964Liu, I., Lawton, L.A. and Robertson, P.K.J. (2003) Mechanistic Studies of the Photo-catalytic Oxidation of Microcystin-LR: An Investigation of Byproducts of the Decomposition Process. Environmental Science & Technology, 37, 3214-3219. https://doi.org/10.1021/es0201855Antoniou, M.G., Shoemaker, J.A., Aa, D.L.C., et al. (2008) Unveiling New Degradation Intermediates/Pathways from the Photocatalytic Degradation of Microcystin-LR. Environmental Science & Technology, 42, 8877-8883.
https://doi.org/10.1021/es801637zTsuji, K., Watanuki, T., Kondo, F., et al. (1997) Stability of Microcystins from Cya-nobacteria—IV. Effect of Chlorination on Decomposition. Toxicon, 35, 1033-1041. https://doi.org/10.1016/S0041-0101(96)00223-1Eleuterio, L. and Batista, J.R. (2010) Biodegradation Studies and Se-quencing of Microcystin-LR Degrading Bacteria Isolated from a Drinking Water Biofilter and a Fresh Water Lake. Toxicon, 55, 1434-1442.
https://doi.org/10.1016/j.toxicon.2010.02.020Jones, G.J., Bourne, D.G., Blakeley, R.L. and Doelle, H. (1994) Degradation of the Cyanobacterial Hepatotoxin Microcystin by Aquatic Bacteria. Natural Toxins, 2, 228-235. https://doi.org/10.1002/nt.2620020412Tsuji, K., Setsuda, S., Watanuki, T., et al. (1996) Microcystin Levels during 1992-95 for Lakes Sagami and Tsukui-Japan. Natural Toxins, 4, 189-194. https://doi.org/10.1002/19960404NT7Cousins, I.T., Bealing, D.J., James, H.A. and Sutton, A. (1996) Biodegradation of Microcystin-LR by Indigenous Mixed Bacterial Populations. Water Research, 30, 481-485. https://doi.org/10.1016/0043-1354(95)00189-1Takenaka, S. and Watanabe, M.F. (1997) Microcystin LR Degradation by Pseudomonas aeruginosa Alkaline Protease. Chemosphere, 34, 749-757. https://doi.org/10.1016/S0045-6535(97)00002-7Park, H.D., Sasaki, Y., Maruyama, T., et al. (2001) Degradation of the Cyanobacterial Hepatotoxin Microcystin by a New Bacterium Isolated from a Hypertrophic Lake. Environmental Toxicology, 16, 337-343.
https://doi.org/10.1002/tox.1041Bourne, D.G., Jones, G.J., Blakeley, R.L., et al. (1996) Enzymatic Pathway for the Bac-terial Degradation of the Cyanobacterial Cyclic Peptide Toxin Microcystin-LR. Applied & Environmental Microbiology, 62, 4086-4094.Babica, P., Bláha, L. and Maršálek, B. (2004) Removal of Microcystins by Phototrophic Biofilms. A Microcosm Study. Environmental Science and Pollution Research, 12, 369-374. https://doi.org/10.1065/espr2005.05.259Saitou, T., Sugiura, N., Itayama, T., Inamori, Y. and Matsumura, M. (2002) Degradation of Microcystin by Biofilm in Practical Treatment Facility. Water Science & Technology, 46, 237-244. https://doi.org/10.2166/wst.2002.0744Li, J., Shimizu, K., Maseda, H., et al. (2012) Investigations into the Biodegradation of Microcystin-LR Mediated by the Biofilm in Wintertime from a Biological Treatment Facility in a Drinking-Water Treatment Plant. Bioresource Technology, 106, 27-35. https://doi.org/10.1016/j.biortech.2011.11.099Wu, Y., Kerr, P.G., Hu, Z. and Yang, L. (2010) Removal of Cyanobacterial Bloom from a Biopond-Wetland System and the Associated Response of Zoobenthic Diversity. Bioresource Technology, 101, 3903-3908.
https://doi.org/10.1016/j.biortech.2009.12.144Liu, X., Huang, S., Tang, T. and Liu, X. (2011) Growth Characteristic and Nitrogen Phosphorous Accumulation Ability of Artificial Wetland Plants. Journal of Water Resources & Water Engineering.