目的:探讨长链非编码RNA(long noncoding RNA, lncRNA)中的lncRNA-19、FER1L4、LINC00152及AK054979在胃癌组织的表达。方法:收集24例胃癌患者癌组织,采用实时荧光定量PCR测定胃癌组织、癌旁组织及正常组织中lncRNA-19、FER1L4、LINC00152及AK054979的表达水平,分析lncRNA-19、FER1L4、LINC00152及AK054979表达与胃癌组织表达的相关性。结果:胃癌组织中lncRNA-19、FER1L4、LINC00152及AK054979的表达水平呈高表达(P均 < 0.01);且与胃癌临床病理分期呈正相关(P < 0.05)。结论:lncRNA-19、FER1L4、LINC00152及AK054979在胃癌组织中均高表达,且与胃癌病理分期呈正相关。
Objective: To explore the expression of lncRNA-19, FER1L4, LINC00152 and AK054979 in long noncoding RNA (lncRNA) in gastric carcinoma. Methods: We collected 24 cases gastric carcinoma tissues. The expression levels of lncRNA-19, FER1L4, LINC00152 and AK054979 in gastric carcinoma tissues, para-cancer tissues and normal tissues were measured by real-time fluorescence quantitative PCR, and the correlation between the expressions of lncRNA-19, FER1L4, LINC00152 and AK054979 and gastric carcinoma tissues was analyzed. Results: lncRNA-19, FER1L4, LINC00152 and AK054979 were highly expressed in gastric cancer tissues (P < 0.01). It was positively correlated with the clinical pathological stages of gastric carcinoma (P < 0.05). Conclusion: lncRNA-19, FER1L4, LINC00152 and AK054979 were all highly expressed in gastric carcinoma tissues and positively correlated with the pathological stages of gastric cancer.
胃癌,长链非编码RNA,lncRNA-19,FER1L4,LINC00152,AK054979, Gastric Cancer Long Non-Coding RNA lncRNA-19 FER1L4 LINC00152 AK054979胃癌病理分期与长链非编码lncRNA表达的相关性研究
Preoperative Borrmann type and pathological manifestations of gastric cance
MSCT大体分型
病理结果
合计
蕈伞型
局限溃疡型
浸润溃疡型
弥漫浸润型
蕈伞型(I)
4
0
0
0
4
局限溃疡型(II)
0
0
2
5
7
浸润溃疡型(III)
1
1
3
4
9
弥漫浸润型(IV)
0
1
1
2
4
合计
5
2
6
11
24
表1. 胃癌术前Borrmann分型与病理表现对照
Results of preoperative enhanced MSCT T staging in 24 patients with gastric cancer were compared with patholog
MSCT分期
病理分期
T1
T2
T3
T4
T1
15
5
0
0
T2
0
7
5
0
T3
0
4
13
6
T4
0
0
6
7
表2. 24例胃癌患者术前增强MSCT T分期结果与病理对照
2.2. 实时荧光定量PCR法检测胃癌组织中lncRNA表达水平
应用TRIzol试剂和AMV反转录试剂盒(均为美国Invitrogen公司产品)提取胃癌组织的总RNA,并将其反转录成cDNA;以此cDNA为模板,应用2 × SYBR Green PCR Master Mix试剂盒(美国Invitrogen公司产品)检测组织中lncRNA RP11-513G11.1的表达情况。
实验步骤
1) RNA提取
将样本分别用液氮研磨粉碎,加入1 ml Trizol (Invitrogen),吸取到1.5 ml EP管中,加入500 ul酚氯仿,振荡混匀,静止5分钟,置于离心机中于4℃离心十分钟,小心吸取上层上清于一新的离心管中,加入700 ul异丙醇,混匀,于4℃ 12,000 rpm离心10分钟,小心去上清,用75%乙醇洗涤一次,晾干,溶于50 ul DEPC处理水中,电泳检测。
高希春,钱 军,魏 铭,徐星榕,汪如荣,辛向斌. 胃癌病理分期与长链非编码lncRNA表达的相关性研究Study on the Correlation between Pathological Staging of Gastric Carcinoma and Expressions of Long-Chain Non-Coding lncRNA[J]. 世界肿瘤研究, 2019, 09(04): 110-117. https://doi.org/10.12677/WJCR.2019.94016
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