胶体金免疫层析试纸条技术(colloidal gold immunochromatography strip, CGIS)作为近年来临床上兴起的新的免疫检验技术,结合了胶体金检测的可视化和免疫层析检测特异性强等特点,因其操作简单迅速、成本较低等特点,适合于临床、实验室、现场及家庭的快速诊断,是目前应用广泛的检验方法,在感染性疾病、自身免疫性疾病等疾病诊断中具有良好的发展前景。本文对胶体金免疫层析试纸条检测技术在疾病诊断中的研究进展进行总结归纳,未来免疫胶体金层析试纸条将向更灵敏特异、多联检测方向进行研究和发展。 Colloidal gold immunochromatography strip (CGIS) is a new immunoassay technique in clinic. It combines the visualization of colloidal gold technique and the specificity of immunochromatography. It has been extensively applied in clinic, laboratory and family with its excellent features at present. It has good prospects for development especially in infectious diseases and autoimmune diseases. This paper will introduce and summarize the development of CGIS technique in the disease diagnosis. Research and development of CGIS will be in the direction of more sensitive specificity and multiplexed detection in the future.
孟 萍. 胶体金免疫层析试纸条在临床诊断中的研究进展Colloidal Gold Immunochromatography Strip Technique and Its Application in Clinical Diagnosis[J]. 纳米技术, 2019, 09(02): 55-59. https://doi.org/10.12677/NAT.2019.92006
参考文献ReferencesFaulk, W.P. and Taylor, G.M. (1971) An Immunocolloid Method for the Electron Microscope. Immunochemistry, 8, 1081-1083. <br>https://doi.org/10.1016/0019-2791(71)90496-4Huang, C.C., Chiang, C.K., Lin, Z.H., Lee, K.H. and Chang, H.T. (2008) Bioconjugated Gold Nanodots and Nanoparticles for Protein Assays Based on Photoluminescence Quenching. Analytical Chemistry, 80, 1497-1504.
<br>https://doi.org/10.1021/ac701998fJain, P.K., Lee, K.S., El-Sayed, I.H. and El-Sayed, M.A. (2006) Calculated Absorption and Scattering Properties of Gold Nanoparticles of Different Size, Shape, and Composition: Applications in Biological Imaging and Biomedicine. The Journal of Physical Chemistry B, 110, 7238-7248. <br>https://doi.org/10.1021/jp057170oVial, S., Pastori-za-Santos, I., Perez-Juste, J. and Liz-Marzan, L.M. (2007) Plasmon Coupling in Layer-by-Layer Assembled Gold Nanorod Films. Langmuir, 23, 4606-4611. <br>https://doi.org/10.1021/la063753tMahato, K., Nagpal, S., Shah, M.A., Srivastava, A., Maurya, P.K., Roy, S., et al. (2019) Gold Nanoparticle Surface Engineering Strategies and Their Applications in Biomedicine and Diagnostics. 3 Biotech, 9, 57.
<br>https://doi.org/10.1007/s13205-019-1577-zZhang, G., Guo, J. and Wang, X. (2009) Immunochromatographic Lateral Flow Strip Tests. Methods in Molecular Biology, 504, 169-183. <br>https://doi.org/10.1007/978-1-60327-569-9_12Scolaro, K.L., Lloyd, K.B. and Helms, K.L. (2008) Devices for Home Evaluation of Women’s Health Concerns. American Journal of Health-System Pharmacy, 65, 299-314. <br>https://doi.org/10.2146/ajhp060565Zhang, G.P., Guo, J.Q., Wang, X.N., Yang, J.X., Yang, Y.Y., Li, Q.M., et al. (2006) Development and Evaluation of an Immunochromatographic Strip for Trichinellosis Detection. Veterinary Parasitology, 137, 286-293.
<br>https://doi.org/10.1016/j.vetpar.2006.01.026Di Nardo, F., Alladio, E., Baggiani, C., Cavalera, S., Giovannoli, C., Spano, G., et al. (2019) Colour-Encoded Lateral Flow Immunoassay for the Simultaneous Detection of Aflatoxin B1 and Type-B Fumonisins in a Single Test Line. Talanta, 192, 288-294. <br>https://doi.org/10.1016/j.talanta.2018.09.037Song, M., Zhang, Y., Li, S., Zhang, C., Tao, M., Tang, Y., et al. (2017) A Sensitive and Rapid Immunoassay for Mycoplasma pneumoniae in Children with Pneumonia Based on Single-Walled Carbon Nanotubes. Scientific Reports, 7, Article No. 16442. <br>https://doi.org/10.1038/s41598-017-16652-3Mdluli, P., Tetyana, P., Sosibo, N., van der Walt, H., Mlambo, M., Skepu, A., et al. (2014) Gold Nanoparticle Based Tuberculosis Immunochromatographic Assay: The Quantitative ESE Quanti Analysis of the Intensity of Test and Control Lines. Biosensors and Bioelectronics, 54, 1-6. <br>https://doi.org/10.1016/j.bios.2013.10.019Blazkova, M., Javurkova, B., Fukal, L. and Rauch, P. (2011) Immunochroma-tographic Strip Test for Detection of Genus Cronobacter. Biosensors and Bioelectronics, 26, 2828-2834. <br>https://doi.org/10.1016/j.bios.2010.10.001Reid, S.M., Ferris, N.P., Bruning, A., Hutchings, G.H., Kowalska, Z. and Aker-blom, L. (2001) Development of a Rapid Chromatographic Strip Test for the Pen-Side Detection of Foot-and-Mouth Disease Virus Antigen. Journal of Virological Methods, 96, 189-202. <br>https://doi.org/10.1016/S0166-0934(01)00334-2Ma, Y., Ni, C., Dzakah, E.E., Wang, H., Kang, K., Tang, S., et al. (2016) Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection. Biomed Research International, 2016, Article ID: 6743904. <br>https://doi.org/10.1155/2016/6743904Yang, S., Yang, J., Zhang, G., Wang, X., Qiao, S., Zhao, D., et al. (2010) Development of an Immunochromatographic Strip for the Detection of Antibodies against Foot-and-Mouth Disease Virus Serotype O. Journal of Virological Methods, 165, 139-144. <br>https://doi.org/10.1016/j.jviromet.2010.01.001Yang, S., Yang, J., Zhang, G., Qiao, S., Wang, X., Zhao, D., et al. (2010) Development of a Peptide-Based Immunochromatographic Strip for Differentiation of Serotype O Foot-and-Mouth Disease Virus-Infected Pigs from Vaccinated Pigs. Journal of Veterinary Diagnostic Investigation, 22, 412-415. <br>https://doi.org/10.1177/104063871002200311Cheng, Y.L., Wang, L.C. and Wang, C.H. (2015) Immunochromatographic Strip Assay Development for Avian Influenza Antibody Detection. Japanese Journal of Veterinary Research, 63, 183-190.Kong, M.M., Yang, B., Gong, C.J., Wang, H., Li, X., Zhao, K.S., et al. (2017) Development of Immu-nochromatographic Colloidal Gold Test Strip for Rapid Detection of Haemophilus influenzae in Clinical Specimens. Journal of Applied Microbiology, 123, 287-294. <br>https://doi.org/10.1111/jam.13489Xiang, T., Jiang, Z., Zheng, J., Lo, C., Tsou, H., Ren, G., et al. (2012) A Novel Double Antibody Sandwich-Lateral Flow Immunoassay for the Rapid and Simple Detection of Hepatitis C Virus. International Journal of Molecular Medicine, 30, 1041-1047. <br>https://doi.org/10.3892/ijmm.2012.1121Sakurai, A., Takayama, K., Nomura, N., Kajiwara, N., Okamatsu, M., Yamamoto, N., et al. (2015) Fluorescent Immunochromatography for Rapid and Sensitive Typing of Seasonal Influenza Viruses. PLoS ONE, 10, e116715.
<br>https://doi.org/10.1371/journal.pone.0116715Sakurai, A., Takayama, K., Nomura, N., Munakata, T., Yamamoto, N., Ta-mura, T., et al. (2013) Broad-Spectrum Detection of H5 Subtype Influenza A Viruses with a New Fluorescent Immunochromatography System. PLoS ONE, 8, e76753. <br>https://doi.org/10.1371/journal.pone.0076753Stenman, U.H., Tiitinen, A., Alfthan, H. and Valmu, L. (2006) The Classification, Functions and Clinical Use of Different Isoforms of HCG. Human Reproduction Update, 12, 769-784. <br>https://doi.org/10.1093/humupd/dml029Tripathi, V., Nara, S., Singh, K., Singh, H. and Shrivastav, T.G. (2012) A Competitive Immunochromatographic Strip Assay for 17-Alpha-Hydroxy Progesterone Using Colloidal Gold Nanoparticles. Clinica Chimica Acta, 413, 262-268.
<br>https://doi.org/10.1016/j.cca.2011.10.016Yang, Q., Gong, X., Song, T., Yang, J., Zhu, S., Li, Y., et al. (2011) Quantum Dot-Based Immunochromatography Test Strip for Rapid, Quantitative and Sensitive Detection of Alpha Fetoprotein. Biosensors and Bioelectronics, 30, 145-150. <br>https://doi.org/10.1016/j.bios.2011.09.002Wu, C.C., Lin, H.Y., Wang, C.P., Lu, L.F., Yu, T.H., Hung, W.C., et al. (2015) Evaluation of a Rapid Quantitative Determination Method of PSA Concentration with Gold Immu-nochromatographic Strips. BMC Urology, 15, 109.
<br>https://doi.org/10.1186/s12894-015-0105-7Wang, C., Hou, F. and Ma, Y. (2015) Simultaneous Quantitative Detection of Multiple Tumor Markers with a Rapid and Sensitive Multicolor Quantum Dots Based Immunochromatographic Test Strip. Biosensors and Bioelectronics, 68, 156-162. <br>https://doi.org/10.1016/j.bios.2014.12.051Chen, Y., Wang, K., Liu, Z., Sun, R., Cui, D. and He, J. (2016) Rapid Detection and Quantification of Tumor Marker Carbohydrate Antigen 72-4 (CA72-4) Using a Superpa-ramagnetic Immunochromatographic Strip. Analytical and Bioanalytical Chemistry, 408, 2319-2327. <br>https://doi.org/10.1007/s00216-016-9328-zAdamcova, M., Sterba, M., Simunek, T., Potacova, A., Popelova, O. and Gersl, V. (2006) Myocardial Regulatory Proteins and Heart Failure. European Journal of Heart Failure, 8, 333-342. <br>https://doi.org/10.1016/j.ejheart.2005.09.007