肝癌是最常见的恶性肿瘤之一,本文的目的是探究天然的茶叶活性成分茶氨酸化学合成获得的衍生物茶乙香酰胺(ethyl coumarin-3-carboxylyl L-theanine, TEC)对人肝癌细胞生长活性的影响。使用化学合成方法由茶氨酸合成茶乙香酰胺,通过细胞生长活性评价方法3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)检测分析不同浓度的TEC作用人肝癌SMMC7721和HepG2细胞48和72小时后的生长活性。实验结果表明:TEC在16-250 µM浓度能够显著性地抑制SMMC7721 (P < 0.01)和HepG2细胞(p < 0.05)的生长,呈现浓度-剂量及时效依赖性作用,并且,TEC对SMMC7721细胞的抑制作用比对HepG2细胞更强。我们目前的结果显示:茶乙香酰胺能够显著性抑制人肝癌SMMC7721细胞和HepG2细胞的生长,值得进一步研究其抗癌活性。 Human hepatocellular carcinoma (HHC) is one of the most common cancers in the world. The purpose of this study is to investigate the effect of ethyl coumarin-3-carboxylyl L-theanine (TEC) synthesized chemically by the theanine from natural tea on the growth of human HHC cell lines. The method of 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) was used to evaluate the effects of TEC at different concentrations on the growth of HHC SMMC7721 and HepG2 cell lines. The experimental results demonstrated that TEC at 16 to 250 μM significantly suppressed the growth of both SMMC7721 (P < 0.01) and HepG2 (P < 0.05) cells at dose- and time-dependent ways after the cells were treated with TEC for 48 and 72h, respectively. The inhibitory effect of TEC on SMMC7721 was much stronger than that of HepG2. Our present results suggest that TEC can significantly repress HHC cell growth and it is worthwhile to further study its anticancer activity.
茶乙香酰胺,肝癌细胞,抗肿瘤, TEC Human Hepatocellular Carcinoma Growth Inhibition茶乙香酰胺对人肝癌细胞生长的影响
张 燕,邓宝玲,石晓玉,邵欣悦,张国营. 茶乙香酰胺对人肝癌细胞生长的影响Effects of Ethyl Coumarin-3-Carboxylyl L-Theanine on the Growth of Human Hepatocellular Carcinoma Cells[J]. 药物化学, 2019, 07(03): 38-44. https://doi.org/10.12677/HJMCe.2019.73007
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