本研究以循环肿瘤细胞(MCF-7)为研究对象,利用软光刻技术制备了微米尺寸的微柱,利用静电纺丝技术在含有微柱结构的基底上面纺上PLGA纳米纤维,形成一种微米和纳米复合尺寸的生物界面,在纳米纤维上修饰特异性抗体,实现肿瘤细胞(MCF-7)的捕获。这种研究方法结合了微流控技术和静电纺丝技术,制备出一种三维微柱网络支架结构,增大了细胞的捕获空间,再通过对微结构上的纳米纤维进行改性,提高了循环肿瘤细胞检测的灵敏度,这种研究方法大大提高了循环肿瘤细胞检测的效率,为临床癌症检测提供了新的研究方向。 In this study, circulating tumor cells (McF-7) were used as the research object. Microcolumns of micron size were prepared by soft photolithography. Using electrostatic spinning technology, PLGA nanofibers were spun on the microcolumnar substrates, then a micron-nanocomposite biological interface was prepared. Specific antibodies were modified on nanofibers, circulating tumor cells can be captured at this time. This research method combines microfluidic technology and electrostatic spinning technology. The three-dimensional microcolumn network scaffold structure is prepared to increase the capture space of cells, and the sensitivity of detection of circulating tumor cells was improved by modifying the nanofibers on the microstructure. This method greatly improves the detection efficiency of circulating tumor cells, and also provides a new research direction for clinical cancer detection.
In this study, circulating tumor cells (McF-7) were used as the research object. Microcolumns of micron size were prepared by soft photolithography. Using electrostatic spinning technology, PLGA nanofibers were spun on the microcolumnar substrates, then a micron-nanocomposite biological interface was prepared. Specific antibodies were modified on nanofibers, circulating tumor cells can be captured at this time. This research method combines microfluidic technology and electrostatic spinning technology. The three-dimensional microcolumn network scaffold structure is prepared to increase the capture space of cells, and the sensitivity of detection of circulating tumor cells was improved by modifying the nanofibers on the microstructure. This method greatly improves the detection efficiency of circulating tumor cells, and also provides a new research direction for clinical cancer detection.
利用静电纺丝机(深圳通力微纳TL-03)在制备的PDMS微芯片微柱表面均匀的纺上PLGA纳米纤维,过程如下:配置15% (w/v)PLGA溶液,取0.9 g PLGA,溶于4.5 mL DMF和1.5 mLTHF混合溶液,均匀搅拌20 min,混合均匀后,将混匀的溶液连接PE管,放置在静电纺丝机中,将静电纺丝机加热器升温至38℃,设置好流速,扫描距离、速度等各项参数后开始纺丝,最后得到纳米纤维,将得到的具有PLGA纳米纤维的PDMS微芯片在扫描电子显微镜(SEM)下成像观察。下图3为不同放大倍数下纺丝后微柱结构的SEM图。可以看到纳米纤维均匀的纺在微柱结构表面并呈线性排列,纳米纤维直径约为350 nm。
张正涛,阮梅林,陈朝会,何荣祥. 功能材料表面修饰用于循环肿瘤细胞的捕获 Functional Materials Surface Modified for the Capture of Circulating Tumor Cells[J]. 纳米技术, 2021, 11(01): 1-7. https://doi.org/10.12677/NAT.2021.111001
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