早期发现、早期诊断是改善肿瘤患者的预后、提高生存率最重要的手段。肿瘤衍生的外泌体是肿瘤早期诊断和预后评估的新型生物标志物。在这里,我们回顾了用于检测肿瘤来源外泌体电化学生物传感器的最新研究进展。主要包括外泌体潜在标志物可用于生物传感器设计的指示性靶标,生物识别元件和信号转换技术。此外,还分析了电化学生物传感技术检测肿瘤来源外泌体所面临的挑战和机遇。
Early detection and diagnosis are the most important means to improve the prognosis and survival rate of tumor patients. Tumor-derived exosomes are new biomarkers for early tumor diagnosis and prognosis evaluation. Here, we review the latest research progress of electrochemical biosensors for detecting tumor-derived exosomes. It mainly includes potential exosomes markers that can be used as indicative targets for biosensor design, biometric identification elements and signal conversion technology. In addition, the challenges and opportunities for the detection of tumor exocrine bodies by electrochemical biosensor technology were also analyzed.
Research Progress of Electrochemical Biosensor for Detecting Exosomes
Junfang Zhu, Wen Xue, Lihong Tang, Jiejing Chen, Yan Chen*
Department of Clinical Laboratory of Guilin No. 924 Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin Guangxi
Received: Mar. 6th, 2021; accepted: Mar. 26th, 2021; published: Apr. 2nd, 2021
ABSTRACT
Early detection and diagnosis are the most important means to improve the prognosis and survival rate of tumor patients. Tumor-derived exosomes are new biomarkers for early tumor diagnosis and prognosis evaluation. Here, we review the latest research progress of electrochemical biosensors for detecting tumor-derived exosomes. It mainly includes potential exosomes markers that can be used as indicative targets for biosensor design, biometric identification elements and signal conversion technology. In addition, the challenges and opportunities for the detection of tumor exocrine bodies by electrochemical biosensor technology were also analyzed.
朱俊芳,薛 雯,唐立红,陈洁晶,陈 燕. 电化学生物传感器检测外泌体的研究进展Research Progress of Electrochemical Biosensor for Detecting Exosomes[J]. 世界肿瘤研究, 2021, 11(02): 33-37. https://doi.org/10.12677/WJCR.2021.112005
参考文献ReferencesTkach, M. and Théry, C. (2016) Communication by Extracellular Vesicles: Where We Are and Where We Need to Go. Cell Actions, 164, 1226-1232. https://doi.org/10.1016/j.cell.2016.01.043郑磊, 李博. 细胞外囊泡生物标志物研究现状与筛选策略[J]. 中华检验杂志, 2018, 41(11): 812-816.郑荣寿, 孙可欣, 张思维, 等. 2015年中国恶性肿瘤流行情况分析[J]. 中华肿瘤杂志, 2019, 41(1): 19-28.Park, S.M., Aalipour, A., Vermesh, O., et al. (2017) Towards Clinically Translatable in Vivo Nanodiagnostics. Nature Reviews Materials, 2, Article No. 17014. https://doi.org/10.1038/natrevmats.2017.14Luppa, P.B., Müller, C., Schlichtiger, A., et al. (2011) Point-of-Care Testing (POCT): Current Techniques and Future Perspectives. Trends in Analytical Chemistry, 30, 887-898. https://doi.org/10.1016/j.trac.2011.01.019Kirsch, J., Siltanen, C., Zhou, Q., et al. (2013) Biosensor Technology: Recent Advances in Threat Agent Detection and Medicine. Chemical Society Reviews, 42, 8733-8768. https://doi.org/10.1039/c3cs60141bAzmi, A.S., Bao, B. and Sarkar, F.H. (2013) Exosomes in Cancer Development, Metastasis, and Drug Resistance: A Comprehensive Review. Cancer and Metastasis Reviews, 32, 623-642. https://doi.org/10.1007/s10555-013-9441-9Kalluri, R. and LeBleu, V.S. (2020) The Biology, Function, and Biomedical Applications of Exosomes. Science, 367, eaau6977. https://doi.org/10.1126/science.aau6977Simpson, R.J., Jensen, S.S. and Lim, J.W.E. (2008) Proteomic Profiling of Exosomes: Current Perspectives. Proteomics, 8, 4083-4099. https://doi.org/10.1002/pmic.200800109Notkins, A.L. (2004) Polyreactivity of Antibody Molecules. Trends in Immunology, 25, 174-179.
https://doi.org/10.1016/j.it.2004.02.004Shangguan, D., Li, Y., Tang, Z., et al. (2006) Aptamers Evolved from Live Cells as Effective Molecular Probes for Cancer Study. Proceedings of the National Academy of Sciences of the United States of America, 103, 11838-11843.
https://doi.org/10.1073/pnas.0602615103Zhou, Q., Rahimian, A., Son, K., et al. (2016) Development of an Aptasensor for Electrochemical Detection of Exosomes. Methods, 97, 88-93. https://doi.org/10.1016/j.ymeth.2015.10.012Gori, A., Romanato, A., Greta, B., et al. (2020) Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles, 9, 1751428. https://doi.org/10.1080/20013078.2020.1751428Gao, X., Ran, N., Dong, X., et al. (2018) Anchor Peptide Captures, Targets, and Loads Exosomes of Diverse Origins for Diagnostics and Therapy. Science Translational Medicine, 10, eaat0195.
https://doi.org/10.1126/scitranslmed.aat0195Wan, Y., Cheng, G., Liu, X., et al. (2017) Rapid Magnetic Isolation of Extracellular Vesicles via Lipid-Based Nanoprobes. Nature Biomedical Engineering, 1, Article No. 0058. https://doi.org/10.1038/s41551-017-0058Zhao, X., Luo, C., Mei, Q., et al. (2020) Aptamer-Cholesterol-Mediated Proximity Ligation Assay for Accurate Identification of Exosomes. Analytical Chemistry, 92, 5411-5418. https://doi.org/10.1021/acs.analchem.0c00141Boriachek, K., Islam, M.N., Gopalan, V., et al. (2017) Quantum Dot-Based Sensitive Detection of Disease Specific Exosome in Serum. Analyst, 142, 2211-2219. https://doi.org/10.1039/C7AN00672AHuang, R., He, L., Xia, Y., et al. (2019) A Sensitive Aptasensor Based on a Hemin/G-Quadruplex-Assisted Signal Amplification Strategy for Electrochemical Detection of Gastric Cancer Exosomes. Small, 15, e1900735.
https://doi.org/10.1002/smll.201900735Zhang, H., Wang, Z., Zhang, Q., et al. (2019) Ti3C2 MXenes Nanosheets Catalyzed Highly Efficient Electrogenerated Chemiluminescence Biosensor for the Detection of Exosomes. Biosensors and Bioelectronics, 124-125, 184-190.
https://doi.org/10.1016/j.bios.2018.10.016Jeong, S., Park, J., Pathania, D., et al. (2016) Integrated Magneto-Electrochemical Sensor for Exosome Analysis. ACS Nano, 10, 1802-1809. https://doi.org/10.1021/acsnano.5b07584Park, J., Lin, H.-Y., Assaker, J.P., et al. (2017) Integrated Kidney Exosome Analysis for the Detection of Kidney Transplant Rejection. ACS Nano, 11, 11041-11046. https://doi.org/10.1021/acsnano.7b05083Doldán, X., Fagúndez, P., Cayota, A., et al. (2016) Electrochemical Sandwich Immunosensor for Determination of Exosomes Based on Surface Marker-Mediated Signal Amplification. Analytical Chemistry, 88, 10466-10473.
https://doi.org/10.1021/acs.analchem.6b02421Wang, S., Zhang, L., Wan, S., et al. (2017) Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical Detection of Cancerous Exosomes. ACS Nano, 11, 3943-3949.
https://doi.org/10.1021/acsnano.7b00373Zhou, Q., Rahimian, A., Son, K., et al. (2016) Development of an Aptasensor for Electrochemical Detection of Exosomes. Methods, 97, 88-93. https://doi.org/10.1016/j.ymeth.2015.10.012Zhou, Y.-G., Mohamadi, R.M., Poudineh, M., et al. (2016) Interrogating Circulating Microsomes and Exosomes Using Metal Nanoparticles. Small, 12, 727-732. https://doi.org/10.1002/smll.201502365