胃癌是常见的恶性肿瘤之一,早期诊断的效果还不理想。近几年研究表明,胃癌患者中一些长链非编码RNA表达异常,可作为胃癌诊断的标志物,治疗及预后监测的指标。本文就长链非编码RNA作为胃癌诊断标志物的研究新进展作一综述。
Gastric cancer is one of the common malignant tumors, and the early diagnosis is not satisfactory. Research in recent years has shown that abnormal expression of some LncRNAs in patients with gastric cancer can be used as markers for diagnosis, treatment and prognostic monitoring of gastric cancer. This article reviews the recent progress of long non-coding RNA as a diagnostic marker for gastric cancer.
胃癌,长链非编码RNA,肿瘤标志物, Gastric Cancer IncRNA Tumor Marker摘要
Gastric cancer is one of the common malignant tumors, and the early diagnosis is not satisfactory. Research in recent years has shown that abnormal expression of some LncRNAs in patients with gastric cancer can be used as markers for diagnosis, treatment and prognostic monitoring of gastric cancer. This article reviews the recent progress of long non-coding RNA as a diagnostic marker for gastric cancer.
Song Meng等 [43] 收集了90位经手术治疗的胃癌患者的血清、胃癌和邻近组织样本以及90位健康成人的血清样本,通过RT-PCR分析LINC01279的表达水平,AUC值为0.82,敏感性和特异性分别为62.2%和95.6%,因此LINC01279的诊断价值比CEA和CA199要高。LINC01279的表达与神经侵袭、血管侵袭、临床阶段、T分类和N分类之间存在显著关联。LINC01279的表达水平与患者的生存时间相关,使用多变量Cox回归分析,高LINC01279表达水平的患者与低LINC01279表达水平的患者相比,生存时间更短。LINC01279的表达与胃癌患者不利的临床特征和不良的预后有关,这表明LINC01279可能通过作为诱因或促进基因而对胃癌起到积极的调节作用。
Sinan Liu等 [47] 用qRT-PCR技术比较了104名GC患者和80名健康对照者的血清RP11-731F5.2水平,发现RP11-731F5.2的表达水平明显高于健康对照,相应的AUC为0.78,敏感性为81.63%,特异性为63.64%。为了验证循环lncRNA的稳定性,将血清在室温下放置24小时,反复冻融7次,血清RP11-731F5.2的表达水平变化不大,说明血清受外界影响较小,相对稳定。此外,用CEA、CA19-9及RP11-731F5.2做了一个联合诊断模型,发现AUC值为0.84,RP11-731F5.2的诊断性能远远优于两个传统生物标志物,联合诊断可以提高诊断价值。同时,胃癌术后样本中血清RP11-731F5.2的水平与术前样本相比明显下降。RP11-731F5.2的水平与患者年龄、性别、肿瘤大小、分期、CEA或CA19-9之间没有相关性。
李蓉蓉. 长链非编码RNA作为胃癌诊断标志物的研究新进展New Progress of Long Non-Coding RNA as a Diagnostic Marker for Gastric Cancer[J]. 临床医学进展, 2022, 12(04): 3635-3640. https://doi.org/10.12677/ACM.2022.124527
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