生物体通过释放各种信号分子来实现细胞间和细胞外信息传递,进而对机体的整体功能进行调节控制,信号分子在生物体中的异常表达会导致多种疾病,因此监测这些生物信号分子对人类健康研究极具意义。三维柔性电化学传感器具有良好的力学性能、灵敏度和稳定性等特性,可以模仿体内细胞的自然环境,实现三维细胞培养和监测。本文简要介绍了三维柔性电化学传感器在细胞生物信号分子监测中的发展和应用。
Organisms transmit intercellular and extracellular information by releasing various signaling molecules, and then regulate and control the overall function of the body. The abnormal expres-sion of signaling molecules in organisms can lead to a variety of diseases, so monitoring these bio-logical signaling molecules is of great significance for human health research.3D flexible electro-chemical sensors have good mechanical properties, sensitivity and stability, which can imitate the natural environment of cells in vivo and realize 3D cell culture. This paper briefly introduces the development and application of 3D flexible electrochemical sensors in Monitoring of Biosignal Molecules.
Organisms transmit intercellular and extracellular information by releasing various signaling molecules, and then regulate and control the overall function of the body. The abnormal expression of signaling molecules in organisms can lead to a variety of diseases, so monitoring these biological signaling molecules is of great significance for human health research.3D flexible electrochemical sensors have good mechanical properties, sensitivity and stability, which can imitate the natural environment of cells in vivo and realize 3D cell culture. This paper briefly introduces the development and application of 3D flexible electrochemical sensors in Monitoring of Biosignal Molecules.
朱彩玲,于春梅,霍晓磊. 三维柔性电化学传感器在细胞信号分子监测中的研究进展Research Progress of Three Dimensional Flexible Electrochemical Sensors in Cell Signal Molecular Monitoring[J]. 分析化学进展, 2022, 12(04): 327-333. https://doi.org/10.12677/AAC.2022.124039
参考文献ReferencesBasudhar, D., Ridnour, L.A., Cheng, R., Kesarwala, A.H., Heinecke, J. and Wink, D.A. (2016) Biological Signaling by Small Inorganic Molecules. Coordination Chemistry Reviews, 306, 708-723. https://doi.org/10.1016/j.ccr.2015.06.001Gao, Y., Zhou, Y. and Chandrawati, R. (2019) Metal and Metal Oxide Nanoparticles to Enhance the Performance of Enzyme-Linked Immunosorbent Assay (ELISA). ACS Applied Nano Materials, 3, 1-21.
https://doi.org/10.1021/acsanm.9b02003Liang, J., Liu, H., Huang, C., et al. (2015) Aggregated Silver Nanoparticles Based Surface-Enhanced Raman Scattering Enzyme-Linked Immunosorbent Assay for Ultrasensitive Detection of Protein Biomarkers and Small Molecules. Analytical Chemistry, 87, 5790-5796. https://doi.org/10.1021/acs.analchem.5b01011Zhen, Z., Liu, J.W., Wen, Q., Wu, Z. and Jiang, J.H. (2020) Homogeneous Label-Free Protein Binding Assay Using Small-Molecule-Labeled DNA Nanomachine with DNAzyme-Based Chemiluminescence Detection. Talanta, 206, Article ID: 120175. https://doi.org/10.1016/j.talanta.2019.120175Gnaim, S. and Shabat, D. (2019) Activity-Based Optical Sensing Enabled by Self-Immolative Scaffolds: Monitoring of Release Events by Fluorescence or Chemilumines-cence Output. Accounts of Chemical Research, 52, 2806-2817.
https://doi.org/10.1021/acs.accounts.9b00338Dal Bello, F., Zorzi, M., Aigotti, R., et al. (2021) Targeted and Untargeted Quantification of Quorum Sensing Signalling Molecules in Bacterial Cultures and Biological Samples via HPLC-TQ MS Techniques. Analytical and Bioanalytical Chemistry, 413, 853-864. https://doi.org/10.1007/s00216-020-03040-6Klencsár, B., Li, S., Balcaen, L. and Vanhaecke, F. (2018) High-Performance Liquid Chromatography Coupled to Inductively Coupled Plasma—Mass Spectrometry (HPLC-ICP-MS) for Quantitative Metabolite Profiling of Non-Metal Drugs. TrAC Trends in Analytical Chemistry, 104, 118-134. https://doi.org/10.1016/j.trac.2017.09.020Menegollo, M., Tessari, I., Bubacco, L. and Szabadkai, G. (2019) Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography in Cultured Cells. Methods in Molecular Biology, 1925, 223-232. https://doi.org/10.1007/978-1-4939-9018-4_19Lopez-Valladares, G., Danielsson-Tham, M.L., Goering, R.V. and Tham, W. (2017) Lineage II (Serovar 1/2a and 1/2c) Human Listeria monocytogenes Pulsed-Field Gel Electrophoresis Types Divided into PFGE Groups Using the Band Patterns Below 145.5 kb. Foodborne Pathogens and Disease, 14, 8-16. https://doi.org/10.1089/fpd.2016.2173Lopez-Canovas, L., Martinez Benitez, M.B., Herrera Isidron, J.A. and Flores Soto, E. (2019) Pulsed Field Gel Electrophoresis: Past, Present, and Future. Ana-lytical Biochemistry, 573, 17-29. https://doi.org/10.1016/j.ab.2019.02.020Zhai, Q. and Cheng, W. (2019) Soft and Stretchable Electrochemical Biosensors. Materials Today Nano, 7, Article ID: 100041. https://doi.org/10.1016/j.mtnano.2019.100041Xu, J., Fang, Y. and Chen, J. (2021) Wearable Biosensors for Non-Invasive Sweat Diagnostics. Biosensors (Basel), 11, Article No. 245. https://doi.org/10.3390/bios11080245Li, D., Wu, C., Tang, X., Zhang, Y. and Wang, T. (2021) Elec-trochemical Sensors Applied for in Vitro Diagnosis. Chemical Research in Chinese Universities, 37, 803-822. https://doi.org/10.1007/s40242-021-0387-0Manickam, P., Vashist, A., Madhu, S., et al. (2020) Gold Nanocubes Embedded Biocompatible Hybrid Hydrogels for Electrochemical Detection of H2O2. Bioelectrochemistry, 131, Article ID: 107373.
https://doi.org/10.1016/j.bioelechem.2019.107373Wang, Y., Li, Y., Zhuang, X., et al. (2021) Ru(bpy)3(2+) Encapsulated Cyclodextrin Based Metal Organic Framework with Improved Biocompatibility for Sensitive Electrochemiluminescence Detection of CYFRA21-1 in Cell. Biosensors and Bioelectronics, 190, Article ID: 113371. https://doi.org/10.1016/j.bios.2021.113371Zhu, X., Xuan, L., Gong, J., et al. (2022) Three-Dimensional Macroscopic Graphene Supported Vertically-Ordered Mesoporous Silica-Nanochannel Film for Direct and Ultrasensitive Detection of Uric Acid in Serum. Talanta, 238, Article ID: 123027. https://doi.org/10.1016/j.talanta.2021.123027Zhang, H.-W., Hu, X.-B., Qin, Y., et al. (2019) Conductive Polymer Coated Scaffold to Integrate 3D Cell Culture with Electrochemical Sensing. Analytical Chemistry, 91, 4838-4844. https://doi.org/10.1021/acs.analchem.9b00478Liu, M.M., Liu, H., Li, S.H., et al. (2021) In-tegrated Paper-Based 3D Platform for Long-Term Cell Culture and in Situ Cell Viability Monitoring of Alzheimer’s Disease Cell Model. Talanta, 223, Article ID: 121738.
https://doi.org/10.1016/j.talanta.2020.121738Liu, Y.L., Qin, Y., Jin, Z.H., et al. (2017) A Stretchable Electrochemical Sensor for Inducing and Monitoring Cell Mechanotransduction in Real Time. Angewandte Chemie International Edition in English, 56, 9454-9458.
https://doi.org/10.1002/anie.201705215Li, J., Su, M., Jiang, M., et al. (2022) Stretchable Conductive Film Based on Silver Nanowires and Carbon Nanotubes for Real-Time Inducing and Monitoring of Cell-Released NO. Sensors and Actuators B—Chemical, 366, Article ID: 131983. https://doi.org/10.1016/j.snb.2022.131983Fan, W.T., Qin, Y., Hu, X.B., et al. (2020) Stretchable Elec-trode Based on Au@Pt Nanotube Networks for Real-Time Monitoring of ROS Signaling in Endothelial Mecha-notransduction. Analytical Chemistry, 92, 15639-15646.
https://doi.org/10.1021/acs.analchem.0c04015Ling, Y., Lyu, Q., Zhai, Q., et al. (2020) Design of Stretchable Holey Gold Biosensing Electrode for Real-Time Cell Monitoring. ACS Sensors, 5, 3165-3171. https://doi.org/10.1021/acssensors.0c01297Fedi, A., Vitale, C., Giannoni, P., Caluori, G. and Marrella, A. (2022) Biosensors to Monitor Cell Activity in 3D Hydrogel-Based Tissue Models. Sensors (Basel), 22, Article No. 1517. https://doi.org/10.3390/s22041517Caviglia, C., Carletto, R.P., De Roni, S., et al. (2020) In Situ Electrochemical Analysis of Alkaline Phosphatase Activity in 3D Cell Cultures. Electrochimica Acta, 359, Article ID: 136951. https://doi.org/10.1016/j.electacta.2020.136951Chu, X., Huang, H., Zhang, H., et al. (2019) Electrochemically Building Three-Dimensional Supramolecular Polymer Hydrogel for Flexible Solid-State Mi-cro-Supercapacitors. Electrochimica Acta, 301, 136-144.
https://doi.org/10.1016/j.electacta.2019.01.165Gao, F., Teng, H., Song, J., Xu, G. and Luo, X. (2020) A Flexible and Highly Sensitive Nitrite Sensor Enabled by Interconnected 3D Porous Polyaniline/Carbon Nanotube Conductive Hydrogels. Analytical Methods, 12, 604-610.
https://doi.org/10.1039/C9AY02442EFeng, Y., Liu, H., Zhu, W., et al. (2021) Muscle-Inspired MXene Conductive Hydrogels with Anisotropy and Low-Temperature Tolerance for Wearable Flexible Sensors and Arrays. Advanced Functional Materials, 31, Article ID: 2105264. https://doi.org/10.1002/adfm.202105264Guo, S., Zhang, C., Yang, M., et al. (2020) A Facile and Sensitive Electrochemical Sensor for Non-Enzymatic Glucose De-tection Based on Three-Dimensional Flexible Polyurethane Sponge Decorated with Nickel Hydroxide. Analytica Chimica Acta, 1109, 130-139. https://doi.org/10.1016/j.aca.2020.02.037Ma, Z., Wei, A., Ma, J., et al. (2018) Lightweight, Compressible and Electrically Conductive Polyurethane Sponges Coated with Synergistic Multiwalled Carbon Nanotubes and Graphene for Piezoresistive Sensors. Nanoscale, 10, 7116-7126. https://doi.org/10.1039/C8NR00004BWen, L., Nie, M., Wang, C., et al. (2021) Multifunctional, Light-Weight Wearable Sensor Based on 3D Porous Polyurethane Sponge Coated with MXene and Carbon Nano-tubes Composites. Advanced Materials Interfaces, 9, Article ID: 2270027. https://doi.org/10.1002/admi.202101592Zhang, J., Sun, Y., Li, X. and Xu, J. (2019) Fabrication of Porous NiMn2O4 Nanosheet Arrays on Nickel Foam as an Advanced Sensor Material for Non-Enzymatic Glucose Detection. Scientific Reports, 9, Article No. 18121.
https://doi.org/10.1038/s41598-019-54746-2Yang, F., Cheng, K., Ye, K., et al. (2014) High Performance of Au Nanothorns Supported on Ni Foam Substrate as the Catalyst for NaBH4 Electrooxidation. Electrochimica Acta, 115, 311-316.
https://doi.org/10.1016/j.electacta.2013.10.110Pang, Y., Tian, H., Tao, L., et al. (2016) Flexible, Highly Sensitive, and Wearable Pressure and Strain Sensors with Graphene Porous Network Structure. ACS Applied Mate-rials & Interfaces, 8, 26458-26462.
https://doi.org/10.1021/acsami.6b08172Deng, Z., Zhao, L., Zhou, H., Xu, X. and Zheng, W. (2022) Recent Advances in Electrochemical Analysis of Hydrogen Peroxide towards in Vivo Detection. Process Biochemistry, 115, 57-69.
https://doi.org/10.1016/j.procbio.2022.01.025Hu, J., Zhang, C., Li, X. and Du, X. (2020) An Electro-chemical Sensor Based on Chalcogenide Molybdenum Disulfide-Gold-Silver Nanocomposite for Detection of Hy-drogen Peroxide Released by Cancer Cells. Sensors (Basel), 20, Article No. 6817. https://doi.org/10.3390/s20236817Fu, Y., Huang, D., Li, C., Zou, L. and Ye, B. (2018) Graphene Blended with SnO2 and Pd-Pt Nanocages for Sensitive Non-Enzymatic Electrochemical Detection of H2O2 Released from Living Cells. Analytica Chimica Acta, 1014, 10-18.
https://doi.org/10.1016/j.aca.2018.01.067Wu, R., Li, L., Pan, L., et al. (2021) Long-Term Cell Culture and Electrically in Situ Monitoring of Living Cells Based on a Polyaniline Hydrogel Sensor. Journal of Materials Chemistry B, 9, 9514-9523.
https://doi.org/10.1039/D1TB01885JBrown, M.D. and Schoenfisch, M.H. (2019) Electrochemical Nitric Oxide Sensors: Principles of Design and Characterization. Chemical Reviews, 119, 11551-11575. https://doi.org/10.1021/acs.chemrev.8b00797Zhao, X., Wang, K., Li, B., et al. (2018) Fabrication of a Flexible and Stretchable Nanostructured Gold Electrode Using a Facile Ultraviolet-Irradiation Approach for the Detection of Nitric Oxide Released from Cells. Analytical Chemistry, 90, 7158-7163. https://doi.org/10.1021/acs.analchem.8b01088Wang, Y.W., Liu, Y.L., Xu, J.Q., Qin, Y. and Huang, W.H. (2018) Stretchable and Photocatalytically Renewable Electrochemical Sensor Based on Sandwich Nanonetworks for Real-Time Monitoring of Cells. Analytical Chemistry, 90, 5977-5981. https://doi.org/10.1021/acs.analchem.8b01396Qin, Y., Hu, X.B., Fan, W.T., et al. (2021) A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real-Time Monitoring of Cells. Advanced Science (Weinh), 8, e2003738.
https://doi.org/10.1002/advs.202003738Shu, Y., Lu, Q., Yuan, F., et al. (2020) Stretchable Electro-chemical Biosensing Platform Based on Ni-MOF Composite/Au Nanoparticle-Coated Carbon Nanotubes for Re-al-Time Monitoring of Dopamine Released from Living Cells. ACS Applied Materials & Interfaces, 12, 49480-49488. https://doi.org/10.1021/acsami.0c16060Li, X., Lu, X. and Kan, X. (2017) 3D Electrochemical Sensor Based on Poly(hydroquinone)/Gold Nanoparticles/Nickel Foam for Dopamine Sensitive Detection. Journal of Elec-troanalytical Chemistry, 799, 451-458.
https://doi.org/10.1016/j.jelechem.2017.06.047Peng, M., Wang, J., Li, Z., et al. (2022) Three-Dimensional Flexible and Stretchable Gold Foam Scaffold for Real-Time Electrochemical Sensing in Cells and in Vivo. Talanta, 253, Article ID: 123891.
https://doi.org/10.1016/j.talanta.2022.123891Liu, Y.L. and Huang, W.H. (2021) Stretchable Electro-chemical Sensors for Cell and Tissue Detection. Angewandte Chemie International Edition in English, 60, 2757-2767. https://doi.org/10.1002/anie.202007754