目前在骨软骨组织工程中支架材料众多,各具有不同功能特性,但尚未找到一种完全代替原有组织的仿生支架材料。但是将各材料通过物理或生物或化学方法进行结合得到的复合材料可弥补其单一支架材料自身不足,将复合材料运用于骨软骨组织工程研究具有良好前景。本文主要就复合材料在骨软骨修复中的作用做一简单综述,报道如下。
At present, there are many scaffold materials in osteochondral tissue engineering, each with dif-ferent functional properties, but a bionic scaffold material that completely replaces the original tissue has not yet been found. However, the composite materials obtained by combining each ma-terial through physical or biological or chemical methods can make up for the shortcomings of single scaffold material itself, and the application of composite materials in osteochondral tissue engineering has good prospects. In this paper, a brief review of the role of composite materials in osteochondral repair is presented and reported as follows.
Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province (Qinghai-Utah Joint Research Key Lab for High Altitude Medicine), Research Center for High Altitude Medicine, Qinghai University, Xining Qinghai
At present, there are many scaffold materials in osteochondral tissue engineering, each with different functional properties, but a bionic scaffold material that completely replaces the original tissue has not yet been found. However, the composite materials obtained by combining each material through physical or biological or chemical methods can make up for the shortcomings of single scaffold material itself, and the application of composite materials in osteochondral tissue engineering has good prospects. In this paper, a brief review of the role of composite materials in osteochondral repair is presented and reported as follows.
李春亮,刘俊豪,熊永斌,何永好,冷梓豪,樊海宁. 壳聚糖、羟基磷灰石、氧化石墨烯材料在骨软骨修复中的研究进展Research Progress of Chitosan, Hydroxyapatite and Graphene Oxide Materials in Osteochondral Repair[J]. 临床医学进展, 2022, 12(11): 10691-10700. https://doi.org/10.12677/ACM.2022.12111540
参考文献ReferencesLiu, J., Fang, Q., Lin, H., Yu, X., Zheng, H. and Wan, Y. (2020) Alginate-Poloxamer/Silk Fibroin Hydrogels with Co-valently and Physically Cross-Linked Networks for Cartilage Tissue Engineering. Carbohydrate Polymers, 247, Article ID: 116593. <br>https://doi.org/10.1016/j.carbpol.2020.116593Yu, J., Lee, S., Choi, S., Kim, K.K., Ryu, B., Kim, C.Y., Jung, C.R., Min, B.H., Xin, Y.Z., Park, S.A., Kim, W., Lee, D. and Lee, J. (2020) Fabrication of a Polycaprolactone/Alginate Bipartite Hybrid Scaffold for Osteochondral Tissue Using a Three-Dimensional Bioprinting System. Polymers, 12, Article No. 2203. <br>https://doi.org/10.3390/polym12102203Nahanmoghadam, A., Asemani, M., Goodarzi, V. and Ebrahimi-Barough, S. (2021) Design and Fabrication of Bone Tissue Scaffolds Based on PCL/PHBV Containing Hydroxyapatite Nanoparticles: Dual-Leaching Technique. Journal of Biomedical Materials Research Part A, 109, 981-993. <br>https://doi.org/10.1002/jbm.a.37087Li, H., Hu, C., Yu, H. and Chen, C. (2018) Chitosan Composite Scaffolds for Articular Cartilage Defect Repair: A Review. RSC Advances, 8, 3736-3749. <br>https://doi.org/10.1039/C7RA11593HSacco, P., Cok, M., Scognamiglio, F., Pizzolitto, C., Vecchies, F., Marfoglia, A., Marsich, E. and Donati, I. (2020) Glycosylated-Chitosan Derivatives: A Systematic Review. Molecules, 25, Article No. 1534.
<br>https://doi.org/10.3390/molecules25071534George, S.M., Nayak, C., Singh, I. and Balani, K. (2022) Multi-functional Hydroxyapatite Composites for Orthopedic Applications: A Review. ACS Biomaterials Science & Engineering, 8, 3162-3186.
<br>https://doi.org/10.1021/acsbiomaterials.2c00140Velasco, M.A., Narváez-Tovar, C.A. and Garzón-Alvarado, D.A. (2015) Design, Materials, and Mechanobiology of Biodegradable Scaffolds for Bone Tissue Engineering. BioMed Research International, 2015, Article ID: 729076.
<br>https://doi.org/10.1155/2015/729076Holt, B.D., Wright, Z.M., Arnold, A.M. and Sydlik, S.A. (2017) Graphene Oxide as a Scaffold for Bone Regeneration. Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, 9, e1437. <br>https://doi.org/10.1002/wnan.1437Bo, L. (2015) Process Aspects in Combus-tion and Gasification Waste-to-Energy (WtE) Units. Waste Management, 37, 13-25. <br>https://doi.org/10.1016/j.wasman.2014.04.019Pighinelli, L. and Kucharska, M. (2013) Chi-tosan-Hydroxyapatite Composites. Carbohydrate Polymers, 93, 256-262.
<br>https://doi.org/10.1016/j.carbpol.2012.06.004Pistone, A., Celesti, C., Piperopoulos, E., Ashok, D., Cembran, A., Tricoli, A. and Nisbet, D. (2019) Engineering of Chitosan-Hydroxyapatite-Magnetite Hierarchical Scaffolds for Guided Bone Growth. Materials, 12, Article No. 2321.
<br>https://doi.org/10.3390/ma12142321Nazeer, M.A., Yilgör, E. and Yilgör, I. (2017) Intercalated Chi-tosan/Hydroxyapatite Nanocomposites: Promising Materials for Bone Tissue Engineering Applications. Carbohydrate Polymers, 175, 38-46.
<br>https://doi.org/10.1016/j.carbpol.2017.07.054Osmond, M.J. and Krebs, M.D. (2021) Tunable Chi-tosan-Calcium Phosphate Composites as Cell-Instructive Dental Pulp Capping Agents. Journal of Biomaterials Science, Polymer Edition, 32, 1450-1465.
<br>https://doi.org/10.1080/09205063.2021.1925390Radwan, N.H., Nasr, M., Ishak, R.A.H., Abdeltawab, N.F. and Awad, G.A.S. (2020) Chitosan-Calcium Phosphate Composite Scaffolds for Control of Post-Operative Osteomye-litis: Fabrication, Characterization, and in vitro-in vivo Evaluation. Carbohydrate Polymers, 244, Article ID: 116482. <br>https://doi.org/10.1016/j.carbpol.2020.116482Boukari, Y., Qutachi, O., Scurr, D.J., Morris, A.P., Doughty, S.W. and Billa, N. (2017) A Dual-Application Poly (Dl-Lactic-co-Glycolic) acid (PLGA)-Chitosan Composite Scaffold for Potential Use in Bone Tissue Engineering. Journal of Biomaterials Science, Polymer Edition, 28, 1966-1983. <br>https://doi.org/10.1080/09205063.2017.1364100Lu, J.Y., He, Y.S., Cheng, C., Wang, Y., Qiu, L., Li, D. and Zou, D.R. (2013) Self-Supporting Graphene Hydrogel Film As an Experimental Platform to Evaluate the Potential of Graphene for Bone Regeneration. Advanced Functional Materials, 23, 3494-3502.Yu, L., Huang, J., et al. (2015) Antler Collagen/Chitosan Scaffolds Improve Critical Calvarial Defect Healing in Rats. Journal of Biomaterials and Tissue Engineering, 5, 774-779. <br>https://doi.org/10.1166/jbt.2015.1368Saravanan, S., Chawla, A., Vairamani, M., Sastry, T.P., Subramanian, K.S. and Selvamurugan, N. (2017) Scaffolds Containing Chitosan, Gelatin and Graphene Oxide for Bone Tissue Regeneration in Vitro and in Vivo. International Journal of Biological Macromolecules, 104, 1975-1985. <br>https://doi.org/10.1016/j.ijbiomac.2017.01.034Chen, Y.H., Tai, H.Y., Fu, E. and Don, T.M. (2019) Guided Bone Regeneration Activity of Different Calcium Phosphate/Chitosan Hybrid Membranes. International Journal of Biological Macromolecules, 126, 159-169.Zhou, K., Yu, P., Shi, X., Ling, T., Zeng, W., Chen, A., Yang, W. and Zhou, Z. (2019) Hierarchically Porous Hydroxyapatite Hybrid Scaffold Incorporated with Reduced Graphene Oxide for Rapid Bone Ingrowth and Repair. ACS Nano, 13, 9595-9606. <br>https://doi.org/10.1021/acsnano.9b04723Cao, H.Q., Zhang, L., Zheng, H. and Wang, Z. (2010) Hydroxyap-atite Nanocrystals for Biomedical Applications. The Journal of Physical Chemistry C, 114, 18352-18357.Di Silvio, L., Gurav, N. and Sambrook, R. (2004) The Fundamentals of Tissue Engineering: New Scaffolds. Medical Journal of Malaysia, 59, 89-90.Khan, S.N., Tomin, E. and Lane, J.M. (2000) Clinical Applications of Bone Graft Substitutes. Orthopedic Clinics of North America, 31, 389-398. <br>https://doi.org/10.1016/S0030-5898(05)70158-9Feng, W, Liang, G, Feng, S, Qi, Y. and Tang, K. (2015) Preparation and Characterization of Collagen-Hydroxyapatite/ Pectin Composite. International Journal of Biological Macromolecules, 74, 218-223.
<br>https://doi.org/10.1016/j.ijbiomac.2014.11.031Song, Y., Wu, H., Gao, Y., Li, J., Lin, K., Liu, B., Lei, X., Cheng, P., Zhang, S., Wang, Y., Sun, J., Bi, L. and Pei. G. (2020) Zinc Silicate/Nano-Hydroxyapatite/Collagen Scaffolds Promote Angiogenesis and Bone Regeneration via the p38 MAPK Pathway in Activated Monocytes. ACS Applied Materials & Interfaces, 12, 16058-16075.
<br>https://doi.org/10.1021/acsami.0c00470Uezono, M., Takakuda, K., Kikuchi, M., Suzuki, S. and Moriyama, K. (2013) Hydroxyapatite/Collagen Nanocomposite-Coated Titanium Rod for Achieving Rapid Osseointegration onto Bone Surface. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 101, 1031-1038. <br>https://doi.org/10.1002/jbm.b.32913Fang, R., Zhang, E., Xu, L. and Wei, S. (2010) Electrospun PCL/PLA/HA Based Nanofibers as Scaffold for Osteoblast-Like Cells. Journal of Nanoscience and Nanotechnology, 10, 7747-7751. <br>https://doi.org/10.1166/jnn.2010.2831Liu, S., Zheng, Y., Liu, R. and Tian, C. (2020) Preparation and Characterization of a Novel Polylactic Acid/Hydroxyapatite Composite Scaffold with Biomimetic Micro-Nanofibrous Porous Structure. Journal of Materials Science: Materials in Medicine, 31, Article No. 74. <br>https://doi.org/10.1007/s10856-020-06415-4Yao, Q., Wei, B., Liu, N., Li, C., Guo, Y., Shamie, A.N., Chen, J., Tang, C., Jin, C., Xu, Y., Bian, X., Zhang, X. and Wang, L. (2015) Chondrogenic Regeneration Using Bone Marrow Clots and a Porous Polycaprolactone-Hydroxyapatite Scaffold by Three-Dimensional Printing. Tissue Engineering Part A, 21, 1388-1397. <br>https://doi.org/10.1089/ten.tea.2014.0280Furtos, G., Rivero, G., Rapuntean, S. and Abraham, G.A. (2017) Amoxicillin-Loaded Electrospun Nanocomposite Membranes for Dental Applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 105, 966-976. <br>https://doi.org/10.1002/jbm.b.33629Freedman, S.L., Banerjee, S., Hocky, G.M. and Dinner, A.R. (2017) Aversatile Framework for Simulating the Dynamic Mechanical Structure of Cytoskeletal Networks. Biophysical Journal, 113, 448-460.Li, .J, Jahr, H., Zheng, W. and Ren, P.G. (2017) Visualizing Angiogenesis by Multiphoton Microscopy in Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair. Journal of Visualized Experiments, 127, e55381. <br>https://doi.org/10.3791/55381Dalgic, A.D., Alshemary, A.Z., Tezcaner, A., Keskin, D. and Evis, Z. (2018) Silicate-Doped Nano-Hydroxyapatite/ Graphene Oxide Composite Reinforced Fibrous Scaffolds for Bone Tissue Engineering. Journal of Biomaterials Applications, 32, 1392-1405. <br>https://doi.org/10.1177/0885328218763665Prakash, J., Prema, D., Venkataprasanna, K.S., Balagangadharan, K., Selvamurugan, N. and Venkatasubbu, G.D. (2020) Nanocomposite Chitosan Film Containing Graphene Ox-ide/Hydroxyapatite/Gold for Bone Tissue Engineering. International Journal of Biological Macromolecules, 154, 62-71. <br>https://doi.org/10.1016/j.ijbiomac.2020.03.095Jiao, D., Zheng, A., Liu, Y., Zhang, X., Wang, X., Wu, J., She, W., Lv, K., Cao, L. and Jiang, X. (2020) Bidirectional Differentiation of BMSCs Induced by a Biomimetic Procallus Based on a Gelatin-Reduced Graphene Oxide Reinforced Hydrogel for Rapid Bone Regeneration. Bioactive Materials, 6, 2011-2028.
<br>https://doi.org/10.1016/j.bioactmat.2020.12.003Qi, Y.Y., Tai, Z.X., Sun, D.F., et al. (2013) Fabrication and Characterization of Poly (Vinyl Alcohol)/Graphene Oxide Nanofibrous Biocomposite Scaffolds. Journal of Applied Polymer Science, 127, 1885-1894.
<br>https://doi.org/10.1002/app.37924Díez-Pascual, A.M. and Díez-Vicente, A.L. (2016) Poly(Propylene Fumarate)/Polyethylene Glycol-Modified Graphene Oxide Nanocomposites for Tissue Engineering. ACS Applied Mate-rials & Interfaces, 8, 7902-7914.
<br>https://doi.org/10.1021/acsami.6b05635Liang, C., Luo, Y., Yang, G., Xia, D., Liu, L., Zhang, X. and Wang, H. (2018) Graphene Oxide Hybridized nHAC/ PLGA Scaffolds Facilitate the Proliferation of MC3T3-E1 Cells. Nanoscale Research Letters, 13, Article No. 15.
<br>https://doi.org/10.1186/s11671-018-2432-6Wang, W., Liu, Y., Yang, C., Qi, X., Li, S., Liu, C. and Li, X. (2019) Mesoporous Bioactive Glass Combined with Graphene Oxide Scaffolds for Bone Repair. International Journal of Biological Sciences, 15, 2156-2169.
<br>https://doi.org/10.7150/ijbs.35670Ahn, J.H., Kim, I.R., Kim, Y., Kim, D.H., Park, S.B., Park, B.S., Bae, M.K., Kim, Y.I. (2020) The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells. Nanomaterials, 10, Article No. 620. <br>https://doi.org/10.3390/nano10040620Xiong, K., Wu, T., Fan, Q., Chen, L. and Yan, M. (2017) Novel Re-duced Graphene Oxide/Zinc Silicate/Calcium Silicate Electroconductive Biocomposite for Stimulating Osteoporotic Bone Regeneration. ACS Applied Materials & Interfaces, 9, 44356-44368. <br>https://doi.org/10.1021/acsami.7b16206Liu, S., Li, Z., Wang, Q., Han, J., Wang, W., Li, S., Liu, H., Guo, S., Zhang, J., Ge, K. and Zhou, G. (2021) Graphene Oxide/Chitosan/Hydroxyapatite Composite Membranes Enhance Os-teoblast Adhesion and Guided Bone Regeneration. ACS Applied Bio Materials, 4, 8049-8059. <br>https://doi.org/10.1021/acsabm.1c00967Ji, M., Li, H., Guo, H., Xie, A., Wang, S., Huang, F., Li, S., Shen, Y. and He, J. (2020) A Novel Porous Aspirin- Loaded (GO/CTS-HA)n Nanocomposite Films: Synthesis and Multifunction for Bone Tissue Engineering. Carbohydrate Polymers, 153, 124-132. <br>https://doi.org/10.1016/j.carbpol.2016.07.078